design-prime-editor-sgrnas

To design pegRNAs for prime editing, provide a target gene symbol or explicit sequence along with the desired edit type (SNV, insertion, or deletion). The tool identifies PE-compatible protospacers, generates PBS and RT templates, and outputs a ranked TSV of guide designs.

pegRNA design supports PE2, PE3, PE3b, and PE-max variants. For PE3 and PE3b, the tool additionally identifies and scores nicking sgRNA candidates to improve editing efficiency alongside the primary pegRNA designs.

Yes, custom PAM sequences are supported when designing prime editing guides. You can specify custom PAMs alongside standard genome builds to identify PE-compatible protospacers on both DNA strands near your desired edit site.

Prime editing guide efficiency is scored by computing PBS melting temperature and evaluating RT template characteristics. The resulting ranked designs are output as a TSV file containing spacers, scores, and optional nicking sgRNA candidates.

Prime editing pegRNA design works for SNV, insertion, and deletion edit types. You supply the edit type and target gene to receive ranked pegRNA designs with PBS sequences, RT templates, and efficiency scores.

Prime editing guide design produces a TSV file with spacer sequences, PBS and RT templates, efficiency scores, and optional nicking sgRNA candidates. It also generates matplotlib plots visualizing score distributions and genomic context.