sgrna-design-guide
CommunityDesign optimal sgRNAs for CRISPR experiments.
Authorjaechang-hits
Version1.0.0
Installs0
System Documentation
What problem does it solve?
This Skill provides a structured decision-making framework to select the most effective sgRNAs for CRISPR experiments, preventing wasted time and resources on poorly designed guides.
Core Features & Use Cases
- Three-Tiered Strategy: Guides users from validated sequences to pre-computed designs to de novo design.
- Enzyme & PAM Matching: Details PAM requirements for SpCas9, SaCas9, AsCas12a, and enAsCas12a.
- Quality Metrics: Explains on-target efficiency, off-target risk, and GC content considerations.
- Application-Specific Rules: Provides targeting rules for knockout, CRISPRa, CRISPRi, base editing, and prime editing.
- Use Case: When planning a CRISPR knockout experiment, use this guide to systematically choose between using a known validated sgRNA from Addgene, selecting a high-ranking guide from the CRISPick database, or designing a new one with CRISPOR if necessary.
Quick Start
Use the sgrna-design-guide skill to find the best sgRNA for a knockout experiment targeting the TP53 gene in human cells.
Dependency Matrix
Required Modules
pandasdbSNP
Components
scriptsreferences
💻 Claude Code Installation
Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.
Please help me install this Skill: Name: sgrna-design-guide Download link: https://github.com/jaechang-hits/SciAgent-Skills/archive/main.zip#sgrna-design-guide Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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