bulkrna-alignment

What problem does it solve?

This Skill addresses the critical need for quality control of bulk RNA-seq count matrices, preventing downstream analysis errors caused by problematic samples.

Core Features & Use Cases

• Library Size Analysis: Assesses total counts per sample to identify library preparation or sequencing issues.
• Gene Detection Rates: Evaluates the number of genes detected in each sample, flagging low-complexity libraries.
• Sample Correlation: Visualizes sample-to-sample similarity to detect batch effects or outlier samples.
• Use Case: Before performing differential expression analysis, run this Skill to ensure your count data is reliable, identify and potentially remove outlier samples, and generate a reproducible QC report.

Quick Start

Run quality control on my bulk RNA-seq count matrix located at 'counts.csv'.