dna-assembly
CommunityDesign DNA assembly primers with confidence.
Software Engineering#bioinformatics#synthetic biology#dna assembly#golden gate#primer design#molecular cloning
AuthorZurybr
Version1.0.0
Installs0
System Documentation
What problem does it solve?
This Skill streamlines the complex process of designing primers for Golden Gate assembly and other multi-fragment DNA assembly workflows, reducing errors and increasing success rates.
Core Features & Use Cases
- Primer Design Guidance: Provides step-by-step instructions for designing primers, including overhang selection and structure.
- Assembly Simulation: Emphasizes the critical step of in-silico simulation to verify assembly outcomes before wet lab work.
- Verification Checklist: Offers a comprehensive checklist to ensure all design parameters are met.
- Use Case: A researcher needs to assemble five DNA fragments into a plasmid using Golden Gate cloning. This Skill guides them through designing the correct overhangs, checking for internal restriction sites, and simulating the final assembly to ensure the correct product is formed.
Quick Start
Use the dna-assembly skill to design primers for a 4-fragment linear Golden Gate assembly.
Dependency Matrix
Required Modules
None requiredComponents
references
💻 Claude Code Installation
Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.
Please help me install this Skill: Name: dna-assembly Download link: https://github.com/Zurybr/lefarma-skills/archive/main.zip#dna-assembly Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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