sequence-analysis

What problem does it solve?

Analyze raw nucleotide or protein sequences quickly to extract key properties, translate coding regions, identify ORFs, map restriction sites, and produce primer suggestions so researchers avoid manual, error-prone sequence inspection.

Core Features & Use Cases

• Sequence properties: length, GC content, composition, complement and reverse complement.
• Translation & ORF finding: translate coding frames and locate open reading frames on both strands.
• Restriction mapping & primer design: detect common enzyme cut sites and propose basic forward/reverse primers with melting temperature estimates.
• Alignment support: perform simple pairwise or basic multiple-sequence alignment when multiple sequences are provided.
• Use case: paste a plasmid or gene sequence to get GC%, translate the coding region, list ORFs above a size threshold, and check for EcoRI/BamHI/HindIII sites before cloning.

Quick Start

Paste the sequence text or FASTA into chat and ask: please analyze this sequence and report length, GC content, translations, ORFs, restriction sites, and propose basic primers.