cell-free-expression
OfficialOptimizes CFPS for higher yield and easier design
Authoradaptyvbio
Version1.0.0
Installs0
System Documentation
What problem does it solve?
This skill guides CFPS optimization by providing a structured framework for planning experiments and diagnosing yield issues.
Core Features & Use Cases
- System selection: guidance across E. coli extracts, wheat germ, PURE, and mammalian-like CFPS platforms.
- Template & codon design: rules for 5' UTR optimization, GC balance, and rare codon management.
- Disulfide formation & folding: strategies to improve oxidation, chaperone use, and fold yield.
- Solubility & stability: fusion tags, buffer additives, and chaperone supplementation to enhance soluble expression.
- Use Case: design a CFPS workflow for a disulfide-rich protein targeting a practical yield with proper folding.
Quick Start
Perform a CFPS planning step by selecting a system (e.g., E. coli extract or Wheat Germ) and outline the optimization tasks needed to increase yield and proper folding.
Dependency Matrix
Required Modules
None requiredComponents
Standard package💻 Claude Code Installation
Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.
Please help me install this Skill: Name: cell-free-expression Download link: https://github.com/adaptyvbio/protein-design-skills/archive/main.zip#cell-free-expression Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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