scanpy-single-cell-analysis
CommunityStreamlined Scanpy workflows for .h5ad data
AuthorKetomihine
Version1.0.0
Installs0
System Documentation
What problem does it solve?
This skill provides a comprehensive, reproducible workflow for analyzing single-cell RNA-seq data stored in .h5ad format using Scanpy within a dedicated conda environment.
Core Features & Use Cases
- Data loading and QC: Efficiently load .h5ad files, assess quality metrics, and filter cells based on gene counts and mitochondrial content.
- Normalization, feature selection, and visualization: Normalize data, identify highly variable genes, and prepare data for downstream analyses, including UMAP visualization.
- Clustering and integration: Construct neighborhood graphs, perform clustering, identify marker genes, and optionally integrate data across batches using scvi-tools for batch correction.
Quick Start
Activate the Scanpy conda environment and run a standard workflow to load an .h5ad file and begin analysis. For example:
- conda activate scanpy
- python -c "import scanpy as sc; adata = sc.read_h5ad('path/to/file.h5ad'); print(adata.shape)"
Dependency Matrix
Required Modules
None requiredComponents
Standard package💻 Claude Code Installation
Recommended: Let Claude install automatically. Simply copy and paste the text below to Claude Code.
Please help me install this Skill: Name: scanpy-single-cell-analysis Download link: https://github.com/Ketomihine/my_skills/archive/main.zip#scanpy-single-cell-analysis Please download this .zip file, extract it, and install it in the .claude/skills/ directory.
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