sarek

Plan and configure nf-core/sarek variant analysis runs from samplesheets.

1|Updated Jun 19, 2026
One-click install
npx skills add https://github.com/danilomonge/nf-claw --skill sarek
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: sarek
Source: https://github.com/danilomonge/nf-claw/tree/main/pipelines/sarek
Command: npx skills add https://github.com/danilomonge/nf-claw --skill sarek

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

nf-core/sarek automates germline and somatic variant analysis so researchers do not need to manually assemble complex Nextflow commands, parameter sets, and reference inputs for every sequencing project.

Core Features & Use Cases

  • End-to-end variant calling: Supports whole-genome, whole-exome, targeted, tumor-normal, and tumor-only workflows from raw reads through annotated outputs.
  • Strict parameter validation: Uses a pinned release schema to constrain inputs, required flags, allowed values, and file formats before the run starts.
  • Practical research use: Helps bioinformatics teams standardize sample sheet-driven analyses, reproduce prior runs, and choose the right tools for alignment, deduplication, calling, and annotation.

Quick Start

Ask for a complete nf-core/sarek run plan for my samplesheet, including the required parameters, recommended step, and the exact nfclaw command for my chosen sequencing scenario.

Frequently Asked Questions about sarek

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I configure an nf-core/sarek pipeline run for tumor-normal sequencing?▼

To configure a tumor-normal sequencing run, provide a validated samplesheet and required parameters to define the workflow. The Skill orchestrates execution planning by constraining inputs and flags against a pinned release schema.

What variant calling workflows can I plan using nf-core/sarek?▼

You can plan germline and somatic variant calling workflows for whole-genome, whole-exome, targeted, tumor-normal, and tumor-only sequencing runs. The Skill validates samplesheet inputs and required parameters for each scenario.

Can I use nf-core/sarek to validate my samplesheet inputs before execution?▼

Yes, nf-core/sarek validates samplesheet inputs, required parameters, allowed values, and file formats before the run starts. It relies on a pinned release schema to constrain inputs and ensure output contracts are met.

How do I get the exact Nextflow command for a whole-genome variant analysis run?▼

Request a complete run plan for your samplesheet to receive the exact Nextflow command. The Skill generates execution configurations including required parameters, recommended steps, and tool-specific run flags.

Does nf-core/sarek support targeted sequencing workflows alongside whole-exome analysis?▼

Yes, nf-core/sarek supports targeted, whole-exome, and whole-genome sequencing workflows. The Skill validates allowed values and run configurations for each sequencing scenario before execution.

Why does my nf-core/sarek run planning fail parameter validation?▼

Run planning fails parameter validation when inputs do not match the pinned release schema. The Skill strictly validates required flags, allowed values, and file formats before generating an execution plan.