bioinformatics-init-analysis

Automate single-cell data analysis from loading through HTML report generation.

Updated Aug 27, 2026
One-click install
npx skills add https://github.com/unstun/dqn10 --skill bioinformatics-init-analysis-unstun
Or copy as Structured Prompt for Agent▼
Please help me install this Agent Skill.
Skill: bioinformatics-init-analysis
Source: https://github.com/unstun/dqn10/tree/main/.claude/skills/bioinformatics-init-analysis
Command: npx skills add https://github.com/unstun/dqn10 --skill bioinformatics-init-analysis-unstun

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires numpy, pandas, scanpy, anndata, fcsparser, scikit-learn, and includes scripts (resource) and references (resource) components.

What problem does it solve?

Automates a full end-to-end analysis pipeline for high-dimensional single-cell biology data, enabling researchers to process CyTOF, scRNA-seq, and flow cytometry with a single pipeline.

Core Features & Use Cases

  • 7-step pipeline: Load → QC → Normalize → PCA/UMAP → Cluster → Marker Analysis → Report
  • Auto-detection of data type with support for CyTOF, scRNA-seq, and flow cytometry files
  • Clinical/plain-language reports and modular execution (full pipeline or individual steps)
  • Real-world use: researchers can run the entire pipeline on a dataset and generate a ready-to-read clinical report

Quick Start

Install the Claude Code plugin and run the full pipeline on your dataset with an input path to auto-detect data type and generate a clinical report.

Frequently Asked Questions about bioinformatics-init-analysis

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I automate scRNA-seq and flow cytometry analysis in a single pipeline?▼

You can automate single-cell data analysis by running a 7-step pipeline that loads data, performs QC and normalization, applies PCA/UMAP, clusters cells, and runs marker analysis to output a processed dataset and clinical HTML report.

Can I run individual steps like clustering and marker analysis separately?▼

Yes, the pipeline supports modular execution, allowing you to run individual steps like clustering or marker analysis independently rather than executing the full end-to-end pipeline at once.

Does this single-cell pipeline support CyTOF and flow cytometry data formats?▼

Yes, the pipeline auto-detects data types and fully supports high-dimensional single-cell biology workflows including CyTOF, scRNA-seq, and flow cytometry files.

How does auto-detection work for scRNA-seq and flow cytometry files?▼

The pipeline automatically detects your single-cell data type by evaluating the input path, applying the appropriate processing logic for scRNA-seq, CyTOF, or flow cytometry without requiring manual configuration.

What is the best way to generate clinical reports from high-dimensional cytometry data?▼

The best way is to use an end-to-end pipeline that processes high-dimensional single-cell data and automatically generates a plain-language HTML clinical report alongside the processed dataset.

Do I need scanpy and anndata installed to process scRNA-seq data?▼

Yes, the pipeline requires scanpy, anndata, numpy, pandas, scikit-learn, and fcsparser to handle data loading, normalization, dimensionality reduction, and clustering across single-cell formats.